Molecular imaging of cancer cells in vivo
AssigneeH. Lee Moffitt Cancer Center and Research Institute, Inc.
InventorsDavid L. Morse, Robert J. Gillies, William Bradford Carter, Narges K. Tafreshi, Marilyn M. Bui, Steven A. Enkemann
Life of this patent
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Abstract
Cellular targets on cancer cells have been identified that can be used with targeted molecular imaging to detect the cancer cells in vivo. Non-invasive methods for detecting cancer cells, such as metastasized cancer cells, are therefore provided. Also provided are compositions and kits for use in the disclosed methods.
Claims11
A non-invasive method for in vivo detection of metastasized breast cancer cells in a subject, the method comprising the steps of:
- (a) administering to the subject a first targeted imaging probe and a second targeted imaging probe,
- wherein the targeted imaging probes each comprise an antibody linked to a NIR fluorophore, wherein the antibody of the first imaging probe specifically binds carbonic anhydrase 9 (CAIX), wherein the antibody of the second imaging probe specifically binds carbonic anhydrase 12 (CAXII);
- and
- (b) imaging the subject with a molecular imaging device to detect the targeted imaging probes in the subject, wherein detection of the targeted imaging probes in an organ or a tissue of the subject is an indication of metastasized breast cancer cells in the organ or the tissue.
A non-invasive method for intraoperative detection of metastasized breast cancer cells in a subject, the method comprising the steps of:
- (a) administering to the subject a first targeted imaging probe and a second targeted imaging probe,
- wherein the targeted imaging probes each comprise an antibody linked to a near-infrared (NIR) fluorophore, and
- wherein the antibody of the first targeted imaging probe specifically binds carbonic anhydrase 9 (CAIX),
- wherein the antibody of the second targeted imaging probe specifically binds carbonic anhydrase 12 (CAXII);
- and
- (b) imaging the subject with a molecular imaging device to detect the targeted imaging probes in the subject,
- wherein detection of the targeted imaging probes in an organ or a tissue of the subject is an indication of metastasized breast cancer cells in the organ or the tissue.
Dependencies are inferred from the claim text.