A method for inducing a humoral immune response in a subject, said method comprising administering to the subject an effective amount of a water-free composition comprising an adjuvant that activates or increases the activity of toll-like receptor 2 (TLR2), dehydrated liposomes, and an antigen, reconstituted in a carrier comprising a continuous phase of a hydrophobic substance,
- wherein the antigen comprises a B cell epitope and is capable of inducing a humoral immune response;
- wherein the adjuvant comprises dipalmitoyl-S-glyceryl-cysteine (PAM2Cys) or tripalmitoyl-S-glyceryl-cysteine (PAM3Cys);
- and wherein the antigen is not coupled covalently to the adjuvant.