Methods of preparing anti-human papillomavirus antigen T cells

A method of treating or preventing HPV+ cancer in a patient, the method comprising:

• (a) culturing multiple fragments of an HPV+ tumor sample from the patient in the presence of at least one cytokine;
• (b) obtaining T-cells from the cultured fragments;
• (c) expanding the number of T-cells to produce an expanded population of HPV-specific T-cells using one or both of (i) irradiated allogenic feeder cells and (ii) irradiated autologous feeder cells;
• and one or both of (iii) OKT3 antibody and (iv) interleukin-2 wherein the T-cells have not been depleted of CD4+ cells;
• (d) optionally, adding a second culturing step following (c);
• and
• (e) administering the expanded population of HPV-specific T-cells to the patient in an amount effective to treat or prevent the HPV+ cancer in the patient.