Method for treating fibromyalgia and chronic fatigue syndrome

A method of treating fibromyalgia (FM) in a subject, comprising:

• (a) determining dysfunction in peripheral blood mononuclear cells (PBMCs), wherein the determining the dysfunction comprises:
• (i) stimulating a sample comprising PBMCs obtained from a subject;
• and
• (ii) measuring expression levels of at least four cytokines selected from the group consisting of IL-2, IL-4, IL5, IL6, IL8, IL 10, IFN-γ, RANTES, MCP-1, MIP-α and MIP-β in a PBMC sample obtained from the subject;
• (b) comparing the expression levels of the at least four cytokines in the PBMC sample obtained from the subject to the expression levels of the at least four cytokines in a control sample of PBMCs;
• (c) diagnosing the subject with FM if dysfunction in PBMCs is detected in the PBMCs obtained from the subject, wherein the dysfunction is indicated by altered expression levels of the at least four cytokines in the PBMC sample obtained from the subject as compared to the expression levels of the at least four cytokines in the control PBMC sample;
• and
• (d) administering a therapeutically effective amount of a vaccine to the subject diagnosed with FM, wherein the vaccine is a live-attenuated or heat-killed Mycobacterium, wherein the Mycobacterium is Mycobacterium bovis (M. bovis), Mycobacterium vaccae (M. vaccae) or Mycobacterium obuense (M. obuense).