Acellular biologic composition and method of manufacture

An implantable acellular freeze-dried biological composition comprising:

• a mixture of mechanically selected biologic material derived and separated from placental tissue wherein the mixture and whole cells taken directly from the placental tissue has the whole cells deliberately removed to form the mixture having non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components;
• wherein the mixture having the non-whole cellular components is further filtered by filtration consisting of filtering through a 0.45 micron filter;
• wherein the mixture is compatible with biologic function to support new tissue formed independent of mesenchymal cells and further includes a select number of non-whole cell fractions including one or more of exosomes, transcriptosomes, proteasomes, membrane rafts, lipid rafts;
• a polyampholyte protectant configured for direct implantation;
• and wherein the composition is freeze-dried and has the mixture added to the polyampholyte protectant at ambient temperature prior to freeze-drying which forms a polyampholyte protectant physical gradient in the mixture and the mixture and polyampholyte protectant is thereafter freeze-dried forming the freeze-dried composition, which after freeze-drying the composition is diluted for direct implantation into a patient.