US 11,076,593 B2 · Reperfusion with omega-3 glycerides promotes donor organ protection for transplantation

Abstract

It has been discovered that isolated organs and tissues perfused/reperfused in perfusion buffer to which omega-3 glyceride oil had been added retain higher levels of function than if perfused/reperfused without the omega-3 glycerides. Isolated hearts reperfused ex vivo after induced ischemia in n-3 triglyceride perfusion emulsion maintained a normal heart rate and normal LVDP and showed a dramatically reduced frequency of arrhythmias compared to control hearts. Further, test hearts reperfused with n-3 oil triglyceride emulsion showed a decrease in creatine kinase and upregulation of certain beneficial proteins including the anti-apoptotic gene marker Bcl-2.

Claims10

Dependent claims

An omega-3 oil in water emulsion application for perfusion of an organ or tissue ex vivo, wherein the emulsion comprises

(a) a perfusion buffer suitable for organ or tissue preservation and transplantation,
(b) from 0.05% to 0.5% of an omega-3 oil by weight in grams per 100 ml of perfusion buffer, wherein the omega-3 oil
(i) comprises from about 10% to about 99% omega-3 diglyceride, omega-3 triglyceride or combinations thereof by weight per total weight of the omega-3 oil, and at least about 20% to about 99% of the total acyl groups of the diglycerides and triglycerides comprise EPA or DHA or combinations thereof, and
(ii) comprises less than 10% omega-6 fatty acids by weight per total weight of the omega-3 oil,
(c) less than 10% omega-6 oil by weight in grams per 100 ml of perfusion buffer, and
(d) the mean diameter of lipid droplets in the emulsion is from about 100 nm to less than about 5 microns.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/113,407Filed 27 Aug 2018 · division

Priority

28 Mar 2013

Published

US 2019/0000072 A13 Jan 2019

Granted

3 Aug 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

A01N 1/126
Preservation or perfusion media: physiologically active agents, e.g. antioxidants or nutrients
A61K 31/202
Having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids: having three or more double…
A01N
Preservation of bodies of humans or animals or plants or parts thereof
A61K
Medicines and medical preparations

Family

Application61/806,391Us provisional application · filed 28 Mar 2013

PCT/US2014/032279Parent of this division · filed 28 Mar 2014

US 2019/0000072 A1Pre-grant publication · published 3 Jan 2019

Application14/780,832Parent of this division

US 10,070,643 B2Parent of this division

US 2016/0050909 A1Published application

US 11,076,593 B2This patent

From the US filing history. Foreign family members aren’t listed.

Reperfusion with omega-3 glycerides promotes donor organ protection for transplantation

Life of this patent

Reperfusion with omega-3 glycerides promotes donor organ protection for transplantation

Life of this patent

Abstract

Claims10