A method of protecting a plant against plant pest, wherein said method comprises applying, to the plant, and/or to the plant pest, a composition comprising a bioactive aqueous extract from the roots of a Parthenium argentatum Gary plant wherein said roots have been separated from the rest of the plant, milled to a size of ¼ inch or less, and extracted by placing them in an aqueous solvent;
- and wherein the milled roots have been removed from the aqueous solvent thereby obtaining the bioactive aqueous extract, and wherein the composition comprises at least 15 compounds selected from ornithine;
- butane-2,3-diol;
- alanine;
- erythritol;
- mannitol;
- lyxitol;
- valine;
- quinic acid;
- 3-hydroxypropionic acid;
- phenylethylamine;
- succinic acid;
- 6-deoxyglucose;
- glycine;
- myo-inositol;
- lactic acid;
- glutamic acid;
- aspartic acid;
- putrescine;
- isoleucine;
- propane-1,3-diol;
- tyrosine;
- oxoproline;
- histidine;
- phosphate;
- 3,4-dihydroxycinnamic acid;
- N-methylalanine;
- uridine;
- ribonic acid;
- glycerol and urea.