IL6R block CAR-T transgenic vector for alleviating CRS, preparation method thereof

The CAR-T transgenic vector of 1, wherein the chimeric antigen receptors of the second-generation CAR comprises:

• a CD8 leader chimeric receptor signal peptide encoded by the sequence of SEQ ID NO:
• 13, a CD19 single chain antibody fragment light chain VL encoded by the sequence of SEQ ID NO:
• 14, an Optimal Linker C encoded by the sequence of SEQ ID NO:
• 15, a CD19 single chain antibody fragment heavy chain VH encoded by the sequence of SEQ ID NO:
• 16, a CD8 Hinge chimeric receptor hinges encoded by the sequence of SEQ ID NO:
• 17, a CD8 transmembrane chimeric receptor transmembrane regions encoded by the sequence of SEQ ID NO:
• 18, a CD137 chimeric receptor costimulatory factors encoded by the sequence of SEQ ID NO:
• 19, and a TCR chimeric receptor T cell activation domains encoded by the sequence of SEQ ID NO:
• 20;
• the chimeric antigen receptors of the third-generation CAR comprises:
• a CD8 leader chimeric receptor signal peptide encoded by the sequence of SEQ ID NO:
• 13, a CD19 single chain antibody fragment light chain VL encoded by the sequence of SEQ ID NO:
• 14, an Optimal Linker C encoded by the sequence of SEQ ID NO:
• 15, a CD19 single chain antibody fragment heavy chain VH encoded by the sequence of SEQ ID NO:
• 16, a CD8 Hinge chimeric receptor hinges encoded by the sequence of SEQ ID NO:
• 17, a CD8 transmembrane chimeric receptor transmembrane regions encoded by the sequence of SEQ ID NO:
• 18, a CD137 chimeric receptor costimulatory factors encoded by the sequence of SEQ ID NO:
• 19, a TCR chimeric receptor T cell activation domains encoded by the sequence of SEQ ID NO:
• 20, and a CD28 chimeric receptor costimulatory factors encoded by the sequence of SEQ ID NO:
• 28.