Compositions and methods for detecting anti-endothelial cell antibodies in allograft rejection

A method of treating an individual who has received an organ transplant, comprising:

• (1) increasing the administration of a therapeutically effective amount of one or more immunosuppressive therapeutics if the individual's antibody expression profile indicates that the individual has an increased risk of developing allograft rejection (AR);
• (2) maintaining the administration of a therapeutically effective amount of one or more immunosuppressive therapeutics if the individual's antibody expression profile indicates that the individual does not have an increased or decreased risk of developing AR;
• or
• (3) decreasing the administration of a therapeutically effective amount of one or more immunosuppressive therapeutics if the individual's antibody expression profile indicates that the individual has a decreased risk of developing AR;
• wherein the antibody expression profile comprises the level of at least four antibodies from a biological sample from the individual that form an affinity interaction with at least a portion or peptide fragment of at least four proteins selected from Table 2;
• and
• wherein a similarity of the level of the at least four antibodies to the positive reference standard indicates increased risk of developing AR and a similarity of the measured level of the at least four antibodies to the negative reference standard indicates decreased risk of developing AR.