Index sorting systems and methods
AssigneeBecton, Dickinson and Company
InventorsDavid A. Roberts, Christopher J. Wolf
Life of this patent
Estimating the term…
Abstract
Features for a quantitative biological data analysis system are described. Index sort analysis can be performed using sort electronics on a flow cytometer or other particle analysis system or after an experiment using a workstation. The sort electronics on the cytometer or other particle analysis system may generate sort decision information for the events. The sort decision information may be transmitted from the sort electronics to the workstation that may differ from the sort identified by the workstation. The feature fuse the gate information with the sorting decision information to provide an accurate representation of the sorting.
Claims22
A computer-implemented method of fused gating for index sorting, the method comprising:
- under control of one or more electronic processors:
- receiving, at a workstation from a memory, experiment information identifying:
- (i) a first sort gate,
- (ii) a first plate destination for particles identified within the first sort gate on a collection plate,
- (iii) a second sort gate, and
- (iv) a second plate destination for particles identified within the second sort gate on the collection plate;
- generating, at the workstation, a first index sort inclusion bitmap identifying first plate locations on the collection plate using the first sort gate;
- generating, at the workstation, a second index sort inclusion bitmap identifying second plate locations on the collection plate using the second sort gate;
- receiving, at the workstation, event data for the particles, the event data including for each particle a fluorescent parameter value and a sort decision of a sorting device;
- determining, via the workstation, that an individual fluorescent parameter value for an individual particle included in the particles is within the first sort gate;
- associating the individual particle with the first sort gate for presentation via a user interface;
- determining, via the workstation, that an individual sort decision for the individual particle corresponds to the second sort gate based at least in part on a comparison between the individual sort decision and the second index sort inclusion bitmap;
- and
- associating the individual particle with the second sort gate for presentation via the user interface.
An apparatus comprising:
- a particle analyzer configured to acquire quantitative biological event data;
- one or more processors in communication with the particle analyzer, the one or more processors configured to:
- receive, from a memory, experiment information identifying:
- (i) a first sort gate,
- (ii) a first plate destination for particles identified within the first sort gate,
- (iii) a second sort gate, and
- (iv) a second plate destination for particles identified within the second sort gate;
- generate a first index sort inclusion bitmap uniquely identifying the first sort gate;
- generate a second index sort inclusion bitmap uniquely identifying and the second sort gate;
- receive, from the particle analyzer, event data for the particles, the event data including for each particle a fluorescent parameter value and a sort decision;
- determine that an individual fluorescent parameter value for an individual particle included in the particles is within the first sort gate;
- associate the individual particle with the first sort gate for presentation via a user interface;
- determine that an individual sort decision for the individual particle corresponds to the second sort gate based at least in part on a comparison between the individual sort decision and the second index sort inclusion bitmap;
- and
- associate the individual particle with the second sort gate for presentation via the user interface.
A non-transitory computer-readable storage medium having stored thereon instructions which when executed by a processor of a device, cause the device to at least:
- receive, from a memory, experiment information identifying:
- (i) a first sort gate,
- (ii) a first plate destination for particles identified within the first sort gate,
- (iii) a second sort gate, and
- (iv) a second plate destination for particles identified within the second sort gate;
- generate a first index sort inclusion bitmap uniquely identifying the first sort gate;
- generate a second index sort inclusion bitmap uniquely identifying and the second sort gate;
- receive, from a particle analyzer, event data for the particles, the event data including for each particle a fluorescent parameter value and a sort decision;
- determine that an individual fluorescent parameter value for an individual particle included in the particles is within the first sort gate;
- associate the individual particle with the first sort gate for presentation via a user interface;
- determine that an individual sort decision for the individual particle corresponds to the second sort gate based at least in part on a comparison between the individual sort decision and the second index sort inclusion bitmap;
- and
- associate the individual particle with the second sort gate for presentation via the user interface.
Dependencies are inferred from the claim text.