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US 11,028,451 B2Granted
Compositions and methods for detection of Mycobacterium tuberculosis
AssigneeRoche Molecular Systems, Inc.
InventorJenny A. Johnson
Life of this patent
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Abstract
Methods for the rapid detection of the presence or absence of Mycobacterium tuberculosis (MTB) and other members of the MTB-complex in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the esxJ gene, along with kits are provided that are designed for the detection of MTB.
Claims10
A kit for detecting a nucleic acid of Mycobacterium tuberculosis (MTB) and other members of the MTB-complex comprising:
- a first primer consisting of SEQ ID NO:
- 1, or the complement thereof, and optionally having at least one modified nucleotide, or SEQ ID NO:
- 7, or the complement thereof, and optionally having at least one modified nucleotide, or SEQ ID NO:
- 8, or the complement thereof, and optionally having at least one modified nucleotide;
- a second primer consisting of SEQ ID NO:
- 15, or the complement thereof, and optionally having at least one modified nucleotide, or SEQ ID NO:
- 16, or the complement, thereof and optionally having at least one modified nucleotide, or SEQ ID NO:
- 17, or the complement thereof, and optionally having at least one modified nucleotide;
- and
- a third fluorescently detectably labeled probe consisting of SEQ ID NO:
- 30, or the complement thereof, and optionally having at least one modified nucleotide, at least one donor fluorescent moiety and at least one corresponding acceptor moiety.
8 more elements
Dependencies are inferred from the claim text.