Assay for detecting hepatitis B virus (HBV)
AssigneeAbbott Molecular Inc.
InventorsDominik Duelli, Wai-Bing Mak, Brian Erickson, Amanda Goldston
Life of this patent
Estimating the term…
Abstract
The disclosure is directed to methods, kits, and compositions, for amplifying and detecting a human hepatitis B virus (HBV) in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes.
Claims9
A set of oligonucleotide sequences for amplifying and detecting a nucleic acid sequence from a hepatitis B virus (HBV) capable of infecting humans in a sample, which comprises:
- (a) a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 1;
- (b) a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 2;
- (c) a first probe oligonucleotide sequence comprising SEQ ID NO:
- 3 and a detectable label;
- (d) a second probe oligonucleotide sequence comprising SEQ ID NO:
- 4 and a detectable label,
- (e) an internal control forward primer oligonucleotide sequence comprising SEQ ID NO:
- 5,
- (f) an internal control reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 6, and
- (g) an internal control probe oligonucleotide sequence comprising SEQ ID NO:
- 7 and a detectable label.
A kit for detecting hepatitis B virus (HBV) capable of infecting humans in a sample comprising:
- (a) a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 1;
- (b) a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 2;
- (c) a first probe oligonucleotide sequence comprising SEQ ID NO:
- 3 and a detectable label;
- (d) a second probe oligonucleotide sequence comprising SEQ ID NO:
- 4 and a detectable label;
- (e) an internal control forward primer oligonucleotide sequence comprising SEQ ID NO:
- 5;
- (f) an internal control reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 6;
- and
- (g) an internal control probe oligonucleotide sequence comprising SEQ ID NO:
- 7 and a detectable label;
- (h) reagents for amplifying and detecting nucleic acid sequences;
- and
- (i) instructions for use.
A composition for amplifying and detecting a hepatitis B virus (HBV) capable of infecting humans in a sample, which comprises:
- (a) a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 1;
- (b) a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 2;
- (c) a first probe oligonucleotide sequence comprising SEQ ID NO:
- 3 and a detectable label;
- and
- (d) a second probe oligonucleotide sequence comprising SEQ ID NO:
- 4 and a detectable label;
- (e) a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 5;
- (f) a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 6;
- and
- (g) a probe oligonucleotide sequence comprising SEQ ID NO:
- 7 and a detectable label.
Dependencies are inferred from the claim text.