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US 11,028,432 B2Granted
Induction PCR
AssigneesBioFire Diagnostics, LLC, University of Utah Research Foundation
InventorsDavid Jones, Carl T. Wittwer
Life of this patent
Estimating the term…
Abstract
Methods, devices, and kits are provided for performing PCR and other thermal cycling reactions in <20 seconds per cycle, using induction heating.
Claims27
A device for thermal cycling a plurality of samples comprising:
- an induction heater and an array of sample wells formed in an electrically conductive material, wherein the induction heater is configured to receive therein the array of sample wells formed in the electrically conductive material, wherein when received, the array is suspended within the induction heater, wherein each of a plurality of the sample wells comprise a target nucleic acid, a thermostable polymerase, and primers configured for amplification of the target nucleic acid sequence, wherein the polymerase is provided at a concentration of at least 0.5 μM and the primers are each provided at a concentration of at least 2 μM,
- a power supply for providing the current to the induction heater, and
- an air source configured to provide air to cool the array of sample wells.
A device for thermal cycling a sample comprising:
- an induction heater and a sample well formed in an electrically conductive material, wherein the induction heater is configured to receive therein the sample well formed in the electrically conductive material, wherein when received, the sample well is suspended within the induction heater, wherein the sample well comprises a target nucleic acid, a thermostable polymerase, and primers configured for amplification of the target nucleic acid sequence, wherein the polymerase and primers are provided at a ratio of (about 0.03 to about 0.4 polymerase):(total primer concentration), and the polymerase concentration is at least 0.5 μM,
- a power supply for providing the current to the induction heater,
- an air source configured to provide air to cool the sample well,
- a switch for controlling the power supply and the fan, and
- a heat sensor for controlling the switch.
2 more elements
Dependencies are inferred from the claim text.