Heat treatment to produce glycosides
AssigneeCargill, Incorporated
InventorsJames C. Anderson, Robert J. Brower, III, Ting Liu Carlson, Belit Flores, Dan S. Gaspard, Kristopher T. Mortenson, Richard Nygaard, Nicole Paulson, Maribeth Rasmussen
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Abstract
Disclosed arc methods of releasing or enriching for steviol glycosides produced by yeast. The disclosed methods enhance or improve the release or enrichment of the steviol glycosides.
Claims21
A method of releasing steviol glycosides from a yeast host cell, comprising the steps of:
- (a) providing a composition, comprising the host cell producing one or more steviol glycosides;
- and
- (b) releasing the one or more steviol glycosides from the host cell by heating the composition to a temperature in a range from 50° C. to 95° C. for 5 minutes to 48 hours, wherein the one or more steviol glycosides are released from an intracellular portion of the host cell into an extracellular portion of the composition.
A composition, comprising a yeast host cell producing one or more steviol glycosides and one or more steviol glycosides that are released from the host cell by heating the composition to a temperature in a range from 50° C. to 95° C. for 5 minutes to 48 hours;
- wherein an amount of the steviol glycosides released extracellularly is in a range of about 80% to about 100% of a total intracellular and extracellular amount of the steviol glycosides produced by the host cell.
Dependencies are inferred from the claim text.