Back to results
US 11,028,413 B2Granted
Microbial engineering for the production of chemical and pharmaceutical products from the isoprenoid pathway
AssigneesMassachusetts Institute of Technology, National University of Singapore
InventorsParayil K. Ajikumar, Gregory Stephanopoulos, Heng Phon Too
Life of this patent
Estimating the term…
Abstract
The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.
Claims22
A method for making a pharmaceutical product, the method comprising:
- providing an Escherichia coli (E. coli) that produces isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) through an upstream methylerythritol pathway (MEP) and converts the IPP and DMAPP to amorphadiene or derivative thereof through a recombinantly expressed downstream synthesis pathway comprising farnesyl diphosphate synthase and amorphadiene synthase;
- culturing the E. coli to produce the amorphadiene or derivative thereof, wherein the accumulation of indole in the culture is controlled to below 100 mg/L to thereby increase production of amorphadiene or derivative thereof;
- and
- incorporating the amorphadiene or derivative thereof, into a pharmaceutical product.
1 more element
A method for making a terpenoid, the method comprising:
- providing an Escherichia coli (E. coli) that produces isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) through an upstream methylerythritol pathway (MEP) and converts the IPP and DMAPP to amorphadiene or derivative thereof through a recombinantly expressed downstream synthesis pathway comprising farnesyl diphosphate synthase and amorphadiene synthase;
- and
- culturing the E. coli to produce the amorphadiene or derivative thereof, wherein the accumulation of indole in the culture is controlled to below 100 mg/L to thereby increase terpenoid production;
- and recovering the amorphadiene or derivative thereof.
1 more element
Dependencies are inferred from the claim text.