Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsAlbert L Lu, Gusui Wu
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Abstract
The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
Claims10
A DNA construct comprising a nucleic acid molecule encoding a variant Cry1B polypeptide having at least 95% sequence identity to SEQ ID NO:
- 29 and having insecticidal activity;
- and a nucleic acid molecule encoding a PtIP-83 polypeptide having at least 95% sequence identity to SEQ ID NO:
- 62, 64, or 66 and having insecticidal activity.
A transgenic plant comprising a molecular stack of a nucleic acid molecule encoding a variant Cry1B polypeptide having at least 95% sequence identity to SEQ ID NO:
- 29 and having insecticidal activity;
- and a nucleic acid molecule encoding a PtIP-83 polypeptide having at least 95% sequence identity to SEQ ID NO:
- 62, 64, or 66 and having insecticidal activity.
A transgenic plant comprising a breeding stack of a nucleic acid molecule encoding a variant Cry1B polypeptide having at least 95% sequence identity to SEQ ID NO:
- 29 and having insecticidal activity;
- and a nucleic acid molecule encoding a PtIP-83 polypeptide having at least 95% sequence identity to SEQ ID NO:
- 62, 64, or 66 and having insecticidal activity.
A composition comprising a nucleic acid molecule encoding a variant Cry1B polypeptide having at least 95% sequence identity to SEQ ID NO:
- 29 and having insecticidal activity and a nucleic acid molecule encoding a PtIP-83 polypeptide having at least 95% sequence identity to SEQ ID NO:
- 62, 64, or 66 and having insecticidal activity.
Dependencies are inferred from the claim text.