US 11,028,402 B2 · Polypeptide and yeast cell compositions and methods of using the same

Abstract

Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.

Claims12

Dependent claims

A polynucleotide encoding a polypeptide comprising at least 95% sequence identity to SEQ ID NO:

4 and comprising at least six amino acid substitutions to SEQ ID NO:
4 at positions selected from the group consisting of S468, I503, G504, N505, V508, I512, N522, F534, L536, V538, and I540;
or a functional fragment of the polypeptide thereof, wherein the polypeptide or functional fragment thereof has maltotriose transporter activity.

Dependencies are inferred from the claim text.

Facts

Application

16/378,106Filed 8 Apr 2019 · us provisional application

Priority

6 Apr 2018

Published

US 2019/0315815 A117 Oct 2019

Granted

8 Jun 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C12C 11/00
Fermentation processes for beer
C12N 15/81
For fungi: for yeasts
C12G 1/0203
Preparation of must from grapes; Must treatment and fermentation: by microbiological or enzymatic treatment
C12G 3/02
Preparation of other alcoholic beverages: by fermentation

Polypeptide and yeast cell compositions and methods of using the same

Life of this patent

Polypeptide and yeast cell compositions and methods of using the same

Life of this patent

Abstract

Claims12