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AssigneeFeed Research Institute Chinese Academy of Agricut
InventorsCanfang Niu, Peilong Yang, Bin Yao, Yangyang Li, Dali Yu, Huiying Luo, Huoqing Huang, Yaru Wang
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The present invention relates to the field of genetic engineering, particularly to phytase variants YkAPPA having amino acid sequence substituting Leucine at the 162th site of the sequence set forth in SEQ ID NO.1 with glycine or proline, or having amino acid sequence substituting glutamic acid at the 230th site of the sequence set forth in SEQ ID NO.1 with glycine, alanine, serine, threonine, aspartic acid, proline, or arginine, and having improved pepsin resistance and increased catalytic efficiency of 2.1 times of that of the wild phytase, in the benefit of the development of economical feed enzyme industry.
A Phytase YkAPPA variant having the amino acid sequence obtained by substituting glutamic acid at the 230th site of the sequence of SEQ ID NO:1 with glycine, proline, or arginine, and having improved pepsin resistance and increased catalytic efficiency.
Dependencies are inferred from the claim text.