Compositions and methods for modulating an immune response
AssigneePresident and Fellows of Harvard College
InventorsPeter T. Sage, Arlene H. Sharpe
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Abstract
The invention provides methods of modulating follicular regulatory T (TFR) cell-mediated immune responses and the use of those methods in the treatment of diseases or conditions such as viral, bacterial, pathogenic, or fungal infections or cancer. Such methods provide for boosting of antibody production through the use of IL-21 to overcome TFR cell suppression of antibody production.
Claims21
A composition comprising T follicular regulatory (TFR) cells and a cytokine, wherein said composition has reduced immune suppressive activity,
- wherein the reduced immune suppressive activity results in a protective antibody response, and
- wherein the cytokine is IL-21 or IL-6.
A method of preparing a composition comprising TFR cells having reduced immune suppressive activity comprising the steps of:
- a) obtaining an initial population of cells comprising TFR cells, T regulatory (Treg) progenitor cells, or both;
- b) contacting the cells ex vivo in the presence of a cytokine;
- and
- c) isolating the TFR cells from the population wherein the isolated TFR cells have reduced immune suppressive activity,
- wherein the reduced immune suppressive activity results in a protective antibody response, and
- wherein the cytokine is IL-21 or IL-6.
A method of modulating an immune response in a subject in need thereof comprising:
- administering to the subject a) an effective amount of a composition comprising T follicular regulatory (TFR) cells and a cytokine, wherein the cytokine is IL-21 or IL-6.
An adjuvant comprising a composition of TFH cells and a cytokine, wherein the adjuvant has an increased protective antibody response, wherein the cytokine is IL-21 or IL-6.
Dependencies are inferred from the claim text.