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US 11,028,362 B2Granted
Decellularized human amniotic membrane for cell delivery, cell culture and inflammation prevention
AssigneeThe Regents of the University of California
InventorsVolodymyr Ryzhuk, Aijun Wang, Diana Farmer, Benjamin Keller
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Abstract
Provided herein is an isolated decelluarized amniotic membrane (DCM) and methods for using same therapeutically in vivo and in vitro. In one aspect, the isolated DCM is further processed, freezing, freeze drying, lyophilization micronized into powder or treatment with pepsin to create a hydrogel.
Claims25
A hydrogel composition comprising:
- a powdered and freeze dried decellularized amniotic membrane (DCM), the amniotic membrane being decellularized using a method comprising:
- adding to the amniotic membrane about a 3% detergent solution comprising t-octylphenoxypolyethoxyethanol solution to lyse the cells,
- rinsing with de-ionized water to remove the detergent solution,
- adding about a 4% sodium deoxycholic acid solution to further decellularize the membrane,
- rinsing with de-ionized water to remove the acid solution,
- adding a solution of about 0.1% peracetic acid/4% ethanol solution to remove residual nucleic acids,
- rinsing with a composition comprising phosphate buffered saline and subsequently de-ionized water, and
- removing excess water;
- stem cells;
- a non-cytotoxic cross-linking agent;
- and
- a carrier comprising buffered saline.
9 more elements
Dependencies are inferred from the claim text.