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US 11,028,358 B2Granted
Cell culture laser photoablation
AssigneeSynthego Corporation
InventorsReed Kelso, Travis Maures
Life of this patent
Estimating the term…
Abstract
Methods and systems for preparing clonal cell populations are described. In some instances the disclosed methods comprise: a) identifying and selecting a cell based on its position on a surface or in a container, where the selection is not based on whether the cell comprises an exogenous label or an expressed reporter; b) photoablating all non-selected cells on the surface or in the container; and c) growing a clonal population of the selected cell.
Claims20
A method comprising:
- a) selecting a cell based on its position on one or more surfaces or in one or more containers, wherein the selecting is not based on whether the cell comprises an exogenous label or an expressed reporter and wherein the selecting is performed using an imaging technique and an automated image analysis process;
- and
- b) photoablating at least 80% of five or more cells on each of the one or more surfaces or in each of the one or more containers, wherein at least 95% of the one or more surfaces or containers contain only one viable cell after the photoablating is performed, wherein the cell selected in (a) is not photoablated.
Dependencies are inferred from the claim text.