Cell culture apparatus and method

A cell culture apparatus comprising:

• a culture column including a first tube-connecting cover, a second tube-connecting cover and at least a scaffold for adherent growth of cells, the first tube-connecting cover, the at least a scaffold and the second tube-connecting cover being sequentially stacked to form a culture column having a closed cavity, and the first tube-connecting cover and the second tube-connecting cover each having a perfusion inlet and a perfusion outlet,
• wherein the culture column further includes a sealing ring and a buckle ring, the sealing ring being arranged around an edge of the scaffold, the buckle ring being arranged around an edge of the sealing ring, wherein a buckle ring boss is provided in an inner circumferential side surface of the buckle ring, a buckle ring slot matching the buckle ring boss is provided in an outer circumferential side surface of the sealing ring, and the sealing rings stacked adjacently are in contact with each other;
• and
• a perfusion system including a culture medium storage apparatus, a perfusion tube and a perfusion pump for powering perfusion, the culture medium storage apparatus, the perfusion pump and the perfusion inlet being sequentially communicated via the perfusion tube.