Bispecific antibody constructs for CDH3 and CD3

A bispecific single chain antibody construct comprising a first human binding domain which binds to an epitope cluster of human CDH3 and macaque CDH3 on the surface of a target cell;

• and
• a second binding domain which binds to human CD3 on the surface of a T cell,
• wherein the epitope cluster of human CDH3 is comprised within amino acid positions 291-363 (SEQ ID NO:
• 36) of human CDH3, and
• wherein the first binding domain comprises a VH region and a VL region selected from the group consisting of:
• a) a VH region comprising a CDR-H1 as depicted in SEQ ID NO:
• 169, a CDR-H2 as depicted in SEQ ID NO:
• 170, and a CDR-H3 as depicted in SEQ ID NO:
• 171, and a VL region comprising a CDR-L1 as depicted in SEQ ID NO:
• 172, a CDR-L2 as depicted in SEQ ID NO:
• 173, and a CDR-L3 as depicted in SEQ ID NO:
• 174;
• b) a VH region comprising a CDR-H1 as depicted in SEQ ID NO:
• 279, a CDR-H2 as depicted in SEQ ID NO:
• 280, and a CDR-H3 as depicted in SEQ ID NO:
• 281, and a VL region comprising a CDR-L1 as depicted in SEQ ID NO:
• 282, a CDR-L2 as depicted in SEQ ID NO:
• 283, and a CDR-L3 as depicted in SEQ ID NO:
• 284;
• c) a VH region comprising a CDR-H1 as depicted in SEQ ID NO:
• 289, a CDR-H2 as depicted in SEQ ID NO:
• 290, and a CDR-H3 as depicted in SEQ ID NO:
• 291, and a VL region comprising a CDR-L1 as depicted in SEQ ID NO:
• 292, a CDR-L2 as depicted in SEQ ID NO:
• 293, and a CDR-L3 as depicted in SEQ ID NO:
• 294;
• d) a VH region comprising a CDR-H1 as depicted in SEQ ID NO:
• 299, a CDR-H2 as depicted in SEQ ID NO:
• 300, and a CDR-H3 as depicted in SEQ ID NO:
• 301, and a VL region comprising a CDR-L1 as depicted in SEQ ID NO:
• 302, a CDR-L2 as depicted in SEQ ID NO:
• 303, and a CDR-L3 as depicted in SEQ ID NO:
• 304;
• e) a VH region comprising a CDR-H1 as depicted in SEQ ID NO:
• 309, a CDR-H2 as depicted in SEQ ID NO:
• 310, and a CDR-H3 as depicted in SEQ ID NO:
• 311, and a VL region comprising a CDR-L1 as depicted in SEQ ID NO:
• 312, a CDR-L2 as depicted in SEQ ID NO:
• 313, and a CDR-L3 as depicted in SEQ ID NO:
• 314.