Recombinant polypeptide production method
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsHisahiro Tabuchi, Tomoya Sugiyama
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Abstract
Provided is a method capable of producing a protein at a high level using a cultured animal cell, comprising culturing a cell that expresses APES (Antibody Production Enhancing Sequence) and into which a DNA encoding a desired polypeptide has been introduced, thereby producing the desired polypeptide. APES contains a nucleotide sequence related to nuclear factor κB inhibitor α (NfkBia) and has a function of decreasing the intracellular expression of NfkBia.
Claims8
A method of producing an antibody comprising culturing a cell into which a DNA encoding a desired antibody and APES (Antibody Production Enhancing Sequence) have been exogenously introduced by transfection, thereby producing the desired antibody, wherein
- the APES is a nucleic acid molecule that increases the ability of the cultured cells to produce the antibody, wherein the APES is selected from nucleic acid molecules each consisting of any of the following nucleotide sequences:
- (a) a DNA consisting of the nucleotide sequence of any of SEQ ID NOs:1 to 16 and 29;
- (b) a DNA consisting of 19 to 25 nucleotides in the sequence of SEQ ID NO:
- 2;
- (c) an RNA that is a transcript of (a), or (b);
- and
- (d) a DNA or RNA consisting of a sequence that can bind 19 to 25 nucleotides in the sequence of SEQ ID NO:
- 2 by base pairing.
Dependencies are inferred from the claim text.