Labelled nucleotides
AssigneeIllumina Cambridge Limited
InventorsXiaohai Liu, John Milton, Silke Ruediger, Xiaolin Wu
Life of this patent
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Abstract
The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).
Claims20
A method for determining the sequence of a single-stranded target polynucleotide, comprising:
- (a) contacting a primer polynucleotide/target polynucleotide complex with one or more of nucleoside triphosphates A, G, C and T, wherein at least one of said nucleoside triphosphate has the structure:
- wherein
- B is a nucleobase selected from the group consisting of a purine, a pyrimidine and a deazapurine;
- Z is —CH2N3;
- X is a triphosphate group;
- Linker-Label comprises
- wherein R is an optionally present electron-withdrawing group;
- Fluor is a fluorophore;
- and the dotted line connecting the Fluor and the benzene ring indicates that substitution may be at any free position on the benzene ring and further atoms not shown maybe present;
- (b) incorporating one nucleoside triphosphate into the primer polynucleotide;
- (c) performing one or more fluorescent measurements to determine the identity of the incorporated nucleoside triphosphate;
- and
- (d) cleaving the Linker after performing the one or more fluorescent measurements.
Dependencies are inferred from the claim text.