Microfluidic cartridge and stacked testing assembly with microfluidic cartridge thereof

A microfluidic cartridge for analyzing fluid samples, comprising:

• a plurality of layers stacked in a height direction;
• wherein
• each of said layers defines a standard number of via ports passing therethrough in the height direction for connecting non-adjacent layers to one another and being closed on the respective layers only if the respective layers need to receive fluid sample from said non-adjacent layers, and a standard number of alignment holes passing therethrough in the height direction and locating at corners thereof to align the layers during assembly of said layers;
• wherein
• at least one of said layers defines a standard number of input ports recessing from one side thereof in the height direction while not penetrating the other opposite side thereof, a standard number of output ports passing therethrough in the height direction and corresponding to the input ports, and flow channels connecting between the respective input ports and output ports;
• said fluid samples enter the layer via the input ports, flowing in the layer via the flow channels and exit of the layer via the output ports;
• wherein
• said layers includes a first layer at least includes said via ports and alignment holes, and a second layer includes said via ports, alignment holes, input ports, output ports and flow channels;
• said first layer stacks on an upper side of the second layer;
• said first layer is an electrode layer and defines a set of electrodes arranged on a lower side thereof;
• said second layer is a counter layer and defines flow channels on an upper side thereof, each flow channel forms a detecting region for said fluid sample passing singly, the electrodes stride across the detecting region to analyze the fluid sample;
• wherein
• the flow channels include a pair of first flow channels and a pair of second flow channels;
• wherein
• said plurality of layers further include another layer stacked on an upper side of the first layer and defining a capture chambers for capturing the selective particles from the fluid sample of interest;
• wherein
• the first layer, the second layer and said another layer are arranged with one another to allow the fluid sample of interest to sequentially pass through the pair of first flow channels in the second layer, the capture chambers in said another layer, and the pair of second flow channels in the second layer.