US 11,027,044 B2 · Method for producing sheet-like cell structure and sheet-like cell structure

Abstract

An object of the present invention is to provide a method for producing a sheet-like cell structure having excellent strength and shape-maintaining performance, and a sheet-like cell structure having excellent strength and shape-maintaining performance. According to the present invention, there is provided a method for producing a sheet-like cell structure, including: a step of adding a biocompatible macromolecular block, a cell, and a liquid medium onto a culture support body having a plurality of recessed portions on a culture surface, and immersing the biocompatible macromolecular block and the cell in uppermost portions of the recessed portions; and a step of culturing the cell to obtain a sheet-like cell structure.

Claims11

Dependent claims

A method for producing a sheet-like cell structure, comprising:

adding a biocompatible macromolecular block, a cell, and a liquid medium onto a culture support body having a plurality of recessed portions on a culture surface, and immersing the biocompatible macromolecular block and the cell in uppermost portions of the recessed portions;
and
culturing the cell to obtain a sheet-like cell structure which maintains its shape and resists rolling;
wherein the culture surface of the culture support body is subjected to a treatment for suppressing adhesion of cells,
the area of the recessed portions on the culture surface is larger than or equal to 70% with respect to the whole area of the culture surface, and
the recessed portions have a depth of from 10 μm to 2,000 μm.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/941,187Filed 30 Mar 2018 · continuation

Priority

JP JP2015-19278530 Sep 2015

Published

US 2018/0280576 A14 Oct 2018

Granted

8 Jun 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification15 codes

A61L 27/38
Containing ingredients of undetermined constitution or reaction products thereof , e.g. transplant tissue, natural bone, extracellular matrix:…
A61L 27/222
Polypeptides or derivatives thereof , e.g. degradation products: gelatin
A61L 27/50
Materials for grafts or prostheses or for coating grafts or prostheses: materials characterised by their function or physical properties , e.g.…
A61L 27/52
Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface…:…

Method for producing sheet-like cell structure and sheet-like cell structure

Life of this patent

Method for producing sheet-like cell structure and sheet-like cell structure

Life of this patent

Abstract

Claims11