PCI method for generating immune respose to antigenic molecule using checkpoint inhibitor and TLR3 ligand

A method of generating an immune response in a subject, comprising administering to a subject an antigenic molecule, a photosensitizing agent, a checkpoint inhibitor and a TLR3 ligand, and irradiating said subject with light of a wavelength effective to activate said photosensitizing agent, wherein an immune response is thereby generated, wherein

• the antigenic molecule is a peptide,
• the checkpoint inhibitor is an anti-CTLA4 antibody, an anti-PD-1 antibody, or both an anti-CTLA4 antibody and an anti-PD-1 antibody, and
• the TLR3 ligand is a double stranded RNA molecule.