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US 11,027,004 B2Granted
Immunogenic compositions containing bacterial outer membrane vesicles
AssigneeBiomvis Srl
InventorsGuido Grandi, Laura Fantappie', Carmela Irene
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Abstract
This invention relates to outer membrane vesicles (OMVs) from Gram-negative bacteria. The vesicles comprise heterologous proteins or immunogenic fragments thereof expressed as lipoproteins in their membrane. The OMVs of the invention are capable of eliciting an immune response to the heterologous protein or to a fragment thereof when administered to a mammal. Other aspects of the invention relate to methods of preparing the OMVs and immunogenic compositions containing the same.
Claims8
A method of preparing an outer membrane vesicle (OMV) from a Gram-negative bacterium, wherein
- said OMV comprises a lipoprotein consisting of a heterologous protein which is a bacterial, viral, parasitic, cancer protein or polypeptide-carrying an acylated N-terminal cysteine,
- said heterologous protein is fused to a lipoprotein leader sequence, said lipoprotein leader sequence causing said heterologous proteins to become lipidated,
- and
- said OMV is capable of eliciting an immune response to the heterologous protein when administered to a mammal,
- said method comprising the following steps:
- (i) expressing, in a Gram-negative bacterium, the heterologous protein fused to said leader sequence carrying a C-terminal Cysteine, wherein the leader sequence comprises the sequence Leu-(Ala/Ser)-(Gly/Ala)-Cys (lipobox), SEQ ID NO:111;
- and
- (ii) isolating the OMV containing the heterologous protein.
5 more elements
Dependencies are inferred from the claim text.