Method for producing haploid, dihaploid and doubled haploid plants by isolated microspore culture

Abstract

The present invention relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid plants of the family Cucurbitaceae from isolated microspores, wherein said method comprises a) culturing isolated microspores to obtain embryos competent for plant regeneration, wherein the microspores have been isolated from plant material of a donor plant of the family Cucurbitaceae; and b) regenerating plants from the embryos; wherein step (a) comprises contacting the microspores with one or more inhibitor of histone deacetylase (HDACi) and one or more polyamine. The present invention also relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid embryos, to related kits and compositions, and to plants obtained according to the methods.

Dependencies are inferred from the claim text.