Use of RNA for reprogramming somatic cells
AssigneesBioNTech SE, TRON—Translationale Onkologie an der Universitätsmedizin der Johannes Gutenberg-Universität Mainz
InventorsUgur Sahin, Marco Poleganov, Tim Beissert
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Abstract
The present invention provides methods for de-differentiating somatic cells into stem-like cells without generating embryos or fetuses. More specifically, the present invention provides methods for effecting the de-differentiation of somatic cells to cells having stem cell characteristics, in particular pluripotency, by introducing RNA encoding factors inducing the de-differentiation of somatic cells into the somatic cells and culturing the somatic cells allowing the cells to de-differentiate.
Claims4
A RNA-transfected somatic cell population which comprises somatic cells subjected to electroporation, containing in vitro transcribed mRNA encoding OCT4, SOX2, KLF4 and c-MYC, wherein the 5′-Cap of said mRNA consists of the D1 isomer of m27,2′-0 GppspG and wherein the transfected cell population expresses alkaline phosphatase and expresses endogenous OCT4, for at least 7 days.
Dependencies are inferred from the claim text.