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US 10,946,088 B2Granted
Preparation of influenza virus vaccine antigens
AssigneeSeqirus UK Limited
InventorsChristoph Haussmann, Frank Hauschild, Bjorn Jobst
Life of this patent
Estimating the term…
Abstract
A number of improvements for preparing vaccine antigens from disintegrated influenza viruses are disclosed. A splitting step can be followed by detergent exchange. Splitting can take place in the presence of a buffer with a higher ionic strength and/or in the presence of phosphate buffer.
Claims22
A method for disrupting influenza virions, the method comprising steps of:
- (i) obtaining a composition comprising influenza virions prepared from a cell culture,
- (ii) inactivating the influenza virions to obtain inactivated virions, and
- (iii) splitting the inactivated virions in the presence of a buffer having an ionic strength of from 200 mM to 800 mM, wherein the buffer comprises phosphate.
Dependencies are inferred from the claim text.