Methods of mediating macrophage phenotypes
AssigneeBiomet Biologics, LLC
InventorsJennifer E. Woodell-May, Joel C. Higgins, William King
Life of this patent
Estimating the term…
Abstract
Methods of inducing a polarization of macrophages. The method includes obtaining a blood fraction, fractionating the blood fraction to produce a blood fraction, and contacting the blood fraction with a source of macrophages. A blood fraction including platelet-poor plasma polarizes the source of macrophages into M1 macrophages. A blood faction including a protein solution polarizes the source of macrophages into M2 macrophages.
Claims20
A method for treating inflammation in a subject, comprising:
- administering to the subject at a site of inflammation a composition comprising between about 1 pg/ml and about 60 pg/ml interleukin-4 (IL-4), wherein monocytes or macrophages at the site of inflammation are induced by the composition to polarize to an M2 phenotype.
A method for treating inflammation in a subject, comprising:
- obtaining a differentiating composition prepared from a tissue and comprising about 1 pg/ml to about 20 pg/ml interleukin-10 (IL-10);
- and
- administering the differentiating composition to a site of inflammation in the subject, wherein the differentiating composition induces monocytes or macrophages at the site of inflammation to differentiate to an M2 phenotype.
A method of treating inflammation in a subject, comprising:
- administering to a site of inflammation in the subject a protein solution prepared from tissue of the subject, the protein solution comprising about 1 pg/ml to about 60 pg/ml interleukin-4 (IL-4) and about 1 pg/ml to about 20 pg/ml interleukin-10 (IL-10) wherein the protein solution induces macrophages or monocytes at the site of inflammation to polarize to an M2 phenotype.
Dependencies are inferred from the claim text.