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US 10,946,035 B2Granted
Production of stable non-polyadenylated RNAs
AssigneeMassachusetts Institute of Technology
InventorsJeremy E. Wilusz, Phillip A. Sharp
Life of this patent
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Abstract
The invention relates in aspects to hybrid RNAs lacking a poly-A tail and nucleic acid vectors for expressing the RNA. The hybrid RNAs in some instances have a stabilizing triple helical structure. Related methods for expressing RNA in vivo and in vitro are also disclosed.
Claims11
A method for expressing an RNA lacking a poly-A tail, comprising:
- expressing in a cell an isolated nucleic acid comprising an RNA having a 3′ heterologous terminal sequence and lacking a poly A tail, wherein the heterologous terminal sequence comprises an RNA stabilizing terminal sequence that has a triple helix conformation, and wherein the immunogenic protein is a viral protein or a cancer protein.
A method for vaccinating a subject, comprising:
- administering to a subject a hybrid nucleic acid comprising an RNA molecule, lacking a poly-A tail, linked to a heterologous RNA stabilizing terminal sequence, wherein the RNA molecule encodes an immunogenic protein, in an effective amount to elicit an adaptive immune response to the immunogenic protein, wherein the heterologous RNA stabilizing terminal sequence has a triple helix conformation.
Dependencies are inferred from the claim text.