ARRDC1-mediated microvesicles (ARMMS) and uses thereof
AssigneesPresident and Fellows of Harvard College, The Board of Trustees of the Leland Stanford Junior University
InventorsQuan Lu, Joseph F. Nabhan, Stanley N. Cohen
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Abstract
The invention provide isolated arrestin domain-containing protein 1 (ARRDC1)-mediated microvesicles (ARMMs). Methods for generating and for isolating ARMMs are also provided herein. ARMMs can be used to deliver agents, for example, nucleic acids (e.g., siRNAs, microRNAs, lincRNAs), proteins (e.g., transcription factors, chromatin modulators, kinases, phosphorylases, or recombinases), or small molecules to target cells in vitro and in vivo, and methods for such ARMM-mediated delivery are provided herein. Diagnostic and therapeutic methods using ARMMs are also described herein.
Claims20
An arrestin domain-containing protein 1 (ARRDC1)-mediated microvesicle (ARMM), comprising
- (a) a lipid bilayer;
- and
- (i) an ARRDC1 protein, or fragment thereof, associated with an enzyme;
- or
- (ii) a TSG101 protein, or fragment thereof, associated with an enzyme.
The microvesicle of 87, wherein the microvesicle diameter is from about 30 nm to about 500 nm.
Dependencies are inferred from the claim text.