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US 10,945,390 B2Granted
Method of selecting corn embryos
AssigneeSyngenta Participations AG
InventorsWilliam Paul Bullock, Ulrich Stephan Hannappel, Katie Marie Hunter
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Abstract
The present invention relates to a viability-retaining method of selecting corn embryos having a desired trait, said method comprising extracting embryos, removing associated non-embryogenic tissue or DNA from the exterior of the embryo; and extracting DNA directly from the embryo. The invention surprisingly shows that germinating embryos sampled directly for their DNA content are capable of continuing the germination process to form normal seedlings.
Claims14
A viability-retaining method of selecting corn embryos having a desired trait, said method comprising:
- a) extracting embryos;
- b) removing associated non-embryogenic tissue or DNA from the exterior of the embryo;
- and
- c) extracting DNA directly from the embryo by contacting the embryos with a cell lysis solution which disrupts cells but does not reduce embryo viability.
1 more element
Dependencies are inferred from the claim text.