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US 10,919,033 B2Granted
Flow cells with hydrogel coating
AssigneesIllumina, Inc., Illumina Cambridge Limited
InventorsHongji Ren, Jonathan Mark Boutell, John A. Moon, M. Shane Bowen, Alex Nemiroski, Gary Mark Skinner, Kenny Chen
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Abstract
In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
Claims5
A flow cell, comprising:
- a patterned substrate including depressions separated by interstitial regions and including a bonding region;
- sequencing surface chemistry attached to each of the depressions, the sequencing surface chemistry including:
- a functionalized coating layer;
- and
- a primer grafted to the functional coating layer;
- a non-grafted hydrogel on at least the sequencing surface chemistry, the non-grafted hydrogel selected from the group consisting of crosslinked polyacrylamide, an agarose gel, and crosslinked polyethylene glycol;
- a lid attached to the patterned substrate at the bonding region;
- and
- a flow channel defined between the patterned substrate and the lid.
6 more elements
Dependencies are inferred from the claim text.