Display substrate, manufacturing method thereof, and display device

A display substrate, comprising:

• a pixel defining layer arranged on a base substrate, wherein a heat-generating material is doped in the pixel defining layer, and the heat-generating material is a material capable of generate heat under a specified condition;
• a first carrier transport layer, a light-emitting material layer and a second carrier transport layer arranged on a side of the pixel defining layer away from the base substrate, wherein an orthographic projection of the second carrier transport layer on the base substrate, and an orthographic projection of the pixel defining layer on the base substrate have an overlapping region, one of the first carrier transport layer and the second carrier transport layer is an electron transport layer, and an other is a hole transport layer;
• wherein each of the first carrier transport layer and the second carrier transport layer has a carrier transport region and a carrier cutoff region, the carrier cutoff region is located in the overlapping region, and a material in the carrier cutoff region is a mixed material of a material of the carrier transport region in the first carrier transport layer and a material of the carrier transport region in the second carrier transport layer.