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US 10,871,640 B2Granted
Methods and systems for automated imaging of three-dimensional objects
AssigneePerkinElmer Cellular Technologies Germany GmbH
InventorsS Chris Hinnah, Kaupo Palo, Ollavi Ollikainen, Thomas Steinkamp, Hartwig Preckel
Life of this patent
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Abstract
Certain configurations are described of methods and systems that can be used to image three-dimensional objects such as biological cells, biological tissues or biological organisms. The methods and systems can image the three-dimensional objects at reduced imaging times and with reduced data volumes.
Claims20
A method of imaging a three-dimensional object present in a matrix using a microscope, the method comprising:
- pre-scanning the matrix using a first magnification objective to identify an x-y location of the three-dimensional object in the matrix and to identify a z-height for the three-dimensional object in the matrix;
- and
- scanning the identified three-dimensional object using a second magnification objective in a z-direction using the identified z-height of the identified three-dimensional object to provide a three-dimensional image of at least some portion of the three-dimensional object present in the matrix, wherein the second magnification objective is greater than or equal to the first magnification objective.
A microscope system configured to image a three-dimensional object in a three-dimensional matrix, the system comprising:
- a sample holder configured to receive a sample comprising the three-dimensional object in the three-dimensional matrix;
- a first light source optically coupled to the sample holder and configured to illuminate at least some portion of the three-dimensional object in the three dimensional matrix that is received by the sample holder, wherein an wavelength provided by the first light source is selected to excite at least one species present in the three-dimensional object;
- at least one objective optically coupled to the light source and configured to receive a light emission from the at least one excited species present in the three-dimensional object in the three-dimensional matrix;
- a detector optically coupled to the at least one objective and configured to receive the light emission from the objective;
- a processor electrically coupled to the detector,
- wherein the microscope system is configured to pre-scan the three-dimensional matrix using a first magnification objective to identify an x-y location of the three-dimensional object and to identify a z-height for the three-dimensional object, and wherein the microscope system is configured use a second magnification objective to scan the identified three-dimensional object in a z-direction using the identified z-height of the identified three-dimensional object to provide a three-dimensional image of at least some portion of the three-dimensional object.
3 more elements
Dependencies are inferred from the claim text.