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US 10,871,485 B2Granted
Kits for labeling of biomarkers and methods of using the same
AssigneeRareCyte, Inc.
InventorsDaniel E. Campton, Yao Sun
Life of this patent
Estimating the term…
Abstract
Kits, compositions, and methods for labeling target materials or target analytes are considered. The composition can include one or more affinity molecules indirectly conjugated to one or more detection moieties. The kit can be used to detect a single biomarker or multiple biomarkers. The kit can be used to perform a single round of labeling or multiple rounds of labeling.
Claims22
A kit comprising:
- a first affinity molecule-first oligonucleotide conjugate;
- a horseradish peroxidase (“HRP”) molecule-second oligonucleotide conjugate;
- and
- a tyramide-first detection moiety conjugate,
- wherein the first and second oligonucleotides are complementary, and
- wherein the tyramide and the first detection moiety are conjugated via complementary third and fourth oligonucleotides.
3 more elements
A kit comprising:
- a first affinity molecule-first oligonucleotide conjugate;
- a horseradish peroxidase (“HRP”) molecule-second oligonucleotide conjugate;
- a tyramide-first detection moiety conjugate,
- a second affinity molecule-third oligonucleotide conjugate;
- a HRP molecule-fourth oligonucleotide conjugate;
- and
- a tyramide-second detection moiety conjugate,
- wherein the first and second oligonucleotides are complementary,
- wherein the third and fourth oligonucleotides are complementary,
- wherein the tyramide and the first detection moiety of the tyramide-first detection moiety conjugate are conjugated via complementary fifth and sixth oligonucleotides;
- and
- wherein the tyramide and the second detection moiety of the tyramide-second detection moiety conjugate are conjugated via complementary seventh and eighth oligonucleotides.
9 more elements
A method for labeling a target analyte of a sample, the method comprising:
- adding, to the sample, a first affinity molecule-first oligonucleotide conjugate;
- adding, to the sample, a horseradish peroxidase (“HRP”) molecule-second oligonucleotide conjugate;
- and
- adding, to the sample, a tyramide-first detection moiety conjugate,
- wherein the first and second oligonucleotides are complementary and hybridize with each other, and
- wherein the tyramide and the first detection moiety are conjugated via complementary third and fourth oligonucleotides.
3 more elements
A method for labeling a target analyte of a sample, the method comprising:
- adding, to the sample, a first affinity molecule-first oligonucleotide conjugate;
- adding, to the sample, a horseradish peroxidase (“HRP”) molecule-second oligonucleotide conjugate;
- adding, to the sample, a tyramide-first detection moiety conjugate,
- adding, to the sample, a second affinity molecule-third oligonucleotide conjugate;
- adding, to the sample, a HRP molecule-fourth oligonucleotide conjugate;
- and
- adding, to the sample, a tyramide-second detection moiety conjugate,
- wherein the first and second oligonucleotides are complementary,
- wherein the third and fourth oligonucleotides are complementary,
- wherein the tyramide and the first detection moiety of the tyramide-first detection moiety conjugate are conjugated via complementary fifth and sixth oligonucleotides;
- and
- wherein the tyramide and the second detection moiety of the tyramide-second detection moiety conjugate are conjugated via complementary seventh and eighth oligonucleotides.
9 more elements
Dependencies are inferred from the claim text.