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US 10,871,484 B2Granted
Enzymatic method for detecting polyaromatic hydrocarbons
AssigneeBioo Scientific Corporation
InventorsNicolas M. Kosa, Joseph F. Krebs, Darshan N. Kasal
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Abstract
Polyaromatic hydrocarbons (PAH) from industrial waste and oil spills are a recurrent threat to food safety due to their stability and toxicity. Existing methods to establish the concentrations of PAH in environmental and food samples rely on expensive and laborious methodology. The teachings herein provide methods and kits for detecting the presence or absence of PAH. In certain embodiments, the quantity of PAH in a sample can be determined.
Claims9
A method for detecting the presence of polyaromatic hydrocarbon (PAH) in a sample, comprising:
- combining a sample with at least one PAH dioxygenase and at least one NAD(P)H cofactor to farm a first reaction solution;
- incubating the first reaction solution under conditions suitable for forming dihydroxydihydro PAH and NAD(P)+;
- adding at least one electron acceptor, at least one electron carrier, and at least one dihydroxy-dihydro PAH dehydrogenase to form a second reaction solution;
- incubating the second reaction solution under conditions suitable for generating a detectable signal;
- and
- measuring the detectable signal, thereby detecting the presence of PAH in the sample.
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Dependencies are inferred from the claim text.