Microfluidic chip device for optical force measurements and cell imaging using microfluidic chip configuration and dynamics

A method for assessing biological particles for use in cell immunotherapy, comprising the use of a device wherein the device comprises:

• the use of a substrate comprising a plurality of channels configured to transport one or more biological particles, wherein the plurality of channels comprises:
• a first channel disposed vertically within the substrate,
• a second channel in operable communication with the first channel and disposed horizontally within the substrate,
• a third channel in operable communication with the second channel and disposed vertically within the substrate, and
• a fourth channel in operable communication with the third channel and disposed horizontally within the substrate;
• wherein the first, second, third and fourth channels are disposed in such a manner as to provide a path for movement of the one or more biological particles through the substrate from the first channel to the second channel to the third channel to the fourth channel,
• injecting one or more biological particles into the first channel disposed vertically within the substrate through a bottom horizontal planar surface having an opening to the first channel, to maintain directional and volumetric continuity with the first channel,
• wherein the biological particles comprise T-cells, engineered T-cells, including CAR T cells,
• assessing the biological particles via optical force-based measurements,
• wherein the optical force-based measurements are used to yield information pertaining to morphology, motility, binding affinities, binding profiles, effect on other biological particles, effect on target cells, susceptibility to external forces such as biological, biochemical, chemical, physical, and temperature influences.