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US 10,871,435 B2Granted
Methods and kits for cell sorting
OwnerShengyuan Yanginventor
Life of this patent
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Abstract
The present disclosure provides methods, and related kits, for directing cell attachment and spreading on a substrate and inducing isotropic spreading of cells; provides methods, and related kits, for cell sorting; and further provides methods, and related kits, for guided induction of stem cell differentiation.
Claims6
A method of cell sorting based on the different adherent sensitivities to substrate curvatures of different cell types, comprising:
- culturing a mixed population of fibroblasts and mesenchymal stem cells on a glass spherical substrate having a diameter of 58-500 μm in cell culture media;
- and
- removing or separating the cell culture media from the glass spherical substrate,
- wherein the fibroblasts attach to the glass spherical substrate after culturing for a sufficient time and the mesenchymal stem cells do not attach to the glass spherical substrate.
1 more element
Dependencies are inferred from the claim text.