Systems and methods for meso-dissection
AssigneeRoche Molecular Systems, Inc.
InventorsMichael Barnes, Christophe Chefd'hotel, Srinivas Chukka, Mohammad Qadri
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Abstract
The subject disclosure presents systems and methods for improved meso-dissection of biological specimens and tissue slides including importing one or more reference slides with annotations, using inter-marker registration algorithms to automatically map the annotations to an image of a milling slide, and dissecting the annotated tissue from the selected regions in the milling slide for analysis, while concurrently tracking the data and analysis using unique identifiers such as bar codes.
Claims15
An instrument for meso-dissection, comprising:
- a processor;
- and a memory coupled to the processor, the memory to store computer-readable instructions that, when executed by the processor, cause the processor to perform operations comprising:
- importing a reference image along with one or more reference annotations, wherein the reference image was digitized from a reference slide scanned on a whole-slide scanner and wherein the one or more reference the annotations were generated using a whole slide viewer interface coupled to the whole-slide scanner;
- automatically registering the one or more reference annotations onto a live capture of a tissue specimen slide to be milled;
- generating a milling annotation based on the one or more reference annotations;
- dissecting tissue from the tissue specimen slide based on the generated milling annotation to provide, a milled tissue sample;
- automatically associating the one or more reference annotations and the milling annotation with the milled tissue sample and with a biological specimen;
- and mapping the milling annotation back to the annotated reference image.
A tangible non-transitory computer-readable medium to store computer-readable code that is executed by a processor to perform operations comprising:
- generating a milling annotation for milling a tissue specimen based on a plurality of reference annotations;
- and milling the tissue specimen using the milling annotation, wherein the milling annotation is generated using an automated intermarker registration algorithm, and wherein the automated intermaker registration algorithm registers an annotation within a whole slide image generated on an external whole-slide scanner on to a live capture of the tissue specimen.
A computer-implemented method for meso-dissection comprising the steps:
- generating a milling annotation for milling a tissue specimen based on a plurality of reference annotations;
- and milling the tissue specimen using the milling annotation, wherein the milling annotation is generated using an automated line-based registration algorithm, and wherein the automated line-based registration algorithm registers an annotation within a whole slide image generated on an external whole-slide scanner on to a live capture of the tissue specimen.
Dependencies are inferred from the claim text.