Dendritic cell response gene expression, compositions of matters and methods of use thereof
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
InventorsAviv Regev, Alexander K. Shalek, Rahul Satija, Hongkun Park
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Abstract
This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences dendritic cell (DC) response(s), for example, dendritic cell maturation, dendritic cell antiviral response(s) and/or dendritic cell inflammatory response(s), as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences dendritic cell response(s) in a variety of therapeutic and/or diagnostic indications.
Claims6
A method of detecting single dendritic cells expressing a maturation gene signature in a subject, comprising:
- detecting increased expression of TMEM39A, or TMEM39A and one or more signature genes or one or more products of one or more signature genes selected from the group consisting of AKNA, APOL7C, APPL1, ARL5C, BATF, BC035044, BCL2L1, BIRC3, BLNK, CCL22, CCR7, CD72, CD80, CD83, CD86, CDKN1A, CHAC2, CRLF3, CSF1, DENND5A, EBI3, EIF2C3, ETS2, ETV3, EXOC3L4, FAM129A, FAM177A1, GPR85, H2-Q7, HSD17B11, IL12B, IL23A, IL4I1, IRF8, ITGA4, KTELC1, LACC1, MKIAA0769, MMP25, NFKBIB, NUDT17, OSGIN2, PALM2, PDZK1IP1, PGAP2, PLAT, PPP1CB, PVR, PVRL2, RAB8B, REL, RHOB, RND3, SAMSN1, SEMA6D, SERPINB9, SRGN, ST3GAL1, STAT3, STAT5A, SWAP70, TBC1D1, TIMP1, TNIP3 and VCAM1 in single dendritic cells of a population of dendritic cells obtained from the subject using single-cell RNA-seq, RNA-fluorescence in situ hybridization (FISH), single-cell quantitative reverse-transcription polymerase chain reaction, fluorescence-activated cell-sorting (FACS), Immunofluorescence (IF), or a combination thereof, wherein the expression is compared to the average expression of the single cells of the population of dendritic cells.
Dependencies are inferred from the claim text.