Method for detecting a presence or absence of at least one first zone of inhibition

A method for detecting a presence or an absence of at least one zone of inhibition, the method comprising:

• obtaining a sample by pre-culturing a crude sample that includes isolating a microorganism strain and incubating the microorganism strain for less than 10 hours so as to increase microorganism biomass;
• providing a determined amount of a chemical agent on a surface of an agar culture medium before or after sample deposition such that the chemical agent defines a potential inhibition zone;
• depositing a volume of the sample in liquid form on the surface of the agar culture medium within a deposition zone extending along an axis at the surface of the agar culture medium so that only a portion of the surface of the agar culture medium is covered with the volume of the sample;
• incubating the agar culture medium with the chemical agent and the volume of the sample on the surface of the agar culture medium;
• and
• determining the presence or absence of the zone of inhibition after incubation, wherein:
• the deposition zone intersects the potential inhibition zone;
• and
• the volume of the sample is deposited in a continuous line.