Methods for producing lipases
AssigneeNovozymes A/S
InventorsLone Baunsgaard, Allan Svendsen, Kim Borch, Rakhi Saikia, Jakob Skjold-Jørgensen
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Abstract
The present invention relates to a method for generating a preparation of a lipase variant of a parent lipase comprising a step of altering one or more nucleotides in a polynucleotide encoding the parent lipase, wherein the alteration provides at least one disulfide bond and a hydrolytic activity of said lipase variant which is reduced as compared with the hydrolytic activity of the parent lipase. The invention also relates to a polynucleotide encoding the lipase variant, the lipase variant and its use.
Claims15
A method for obtaining a lipase variant of a parent lipase, comprising introducing into a parent lipase an alteration at one or more positions of the parent lipase, wherein the alteration at the one or more positions provides at least one disulfide bond and wherein hydrolytic activity of the lipase variant is reduced as compared to hydrolytic activity of the parent lipase,
- wherein the alteration is selected from one or more amino acid substitutions selected from the group consisting of L57C/N120C, L54C/P101C, A47C/V102C, S24C/G104C, P46C/N186C, S43C/N186C, T40C/P182C, T40C/L216C, T40C/L239C, D38C/A212C, P37C/E240C, P37C/N246C, E36C/N246C, E36C/V249C, A33C/V249C and S31C/V249C, wherein the position corresponds to the positions of SEQ ID NO:
- 2
- and wherein the amino acid sequence of the lipase variant has at least 90% sequence identity with SEQ ID NO:
- 2.
A lipase variant of a parent lipase, comprising an alteration at one or more positions compared to the parent lipase, wherein the alteration provides at least one disulfide bond and wherein hydrolytic activity of said lipase variant is reduced as compared with hydrolytic activity of the parent lipase,
- wherein the variant comprises one or more amino acid substitutions selected from the group consisting of L57C/N120C, L54C/P101C, A47C/V102C, S24C/G104C, P46C/N186C, S43C/N186C, T40C/P182C, T40C/L216C, T40C/L239C, D38C/A212C, P37C/E240C, P37C/N246C, E36C/N246C, E36C/V249C, A33C/V249C and S31C/V249C, wherein the positions correspond to the positions of SEQ ID NO:
- 2,
- and wherein the amino acid sequence of the lipase variant has at least 90% sequence identity with SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.