Method for separating cell from biological tissue

A method for separating pancreatic islets from a pancreas tissue, using a degrading-enzyme composition, comprising:

• measuring an abundance of collagen I and collagen III in the pancreas tissue,
• determining an amount ratio of collagenase H to collagenase G derived from Clostridium sp. based on the measured abundance of collagen I and collagen III, wherein a higher amount ratio is used when the measured abundance is higher and a lower amount ratio is used when the measured abundance is lower,
• preparing the degrading-enzyme composition by adding collagenase H and collagenase G derived from Clostridium sp. with the determined ratio, to a predetermined amount of one or more of a neutral protease or a protease derived from Clostridium sp.,
• treating the pancreas tissue with the degrading-enzyme composition, and
• separating pancreatic islets from the pancreas tissue treated with the degrading-enzyme composition,
• wherein collagenase H degrades collagen I and collagen III, and
• wherein the amount ratio (H/G) of collagenase H to collagenase G in the degrading-enzyme composition is 0.35 or more.