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US 10,870,831 B2Granted
Method for inducing differentiation of neural stem cells using patterned hydrogel
AssigneeSogang University Research Foundation
InventorsBong Geun Chung, Jong Min Lee, Joo Yoon Moon, Tae Hyeon Kim, Daniel Christian Ahrberg
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Abstract
The present invention provides a method for inducing differentiation of neural stem cells. The present invention provides optimized differentiation conditions of neural stem cells into neurons using a patterned hydrogel.
Claims7
A method for inducing differentiation of neural stem cells, the method comprising:
- (a) culturing neural stem cells to induce neurospheres;
- (b) seeding the neurospheres on a cell culture substrate on which a hydrogel comprising a biocompatible polymer and a conductive nanowire is patterned to have intervals of 150-250 μm;
- and
- (c) applying voltage to the cell culture substrate to induce the differentiation into neurons,
- wherein the conductive nanowires are patterned by mixing with the biocompatible polymer, and fixed by photocrosslinking of the biocompatible polymer, and
- wherein the hydrogel in step (b) comprises a biocompatible polymer and a conductive nanowire at a mixing ratio of 8-12:1.
3 more elements
Dependencies are inferred from the claim text.