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US 10,870,824 B2Granted
Cell support composite and method for producing cell support composite
AssigneeNikkiso Co., Ltd.
InventorFumihiko Kitagawa
Life of this patent
Estimating the term…
Abstract
A cell support composite includes: a substrate formed of an artificial material; a laminin molecule or a fragment thereof which adheres to at least a part of the substrate; and a renal tubule epithelial cell or a renal tubule epithelial-like cell which is a culture cell attached to the substrate via the laminin molecule or the fragment thereof.
Claims6
A cell support composite comprising:
- a substrate formed of an artificial material;
- a laminin molecule or a fragment thereof which adheres to at least a part of the substrate;
- and
- a monolayer of a renal tubule epithelial cell or a renal tubule epithelial-like cell which is a cultured cell attached to the substrate via the laminin molecule or the fragment thereof, wherein the monolayer of the renal tubule epithelial cell or the renal tubule epithelial-like cell is maintained for 15 days or more,
- wherein the laminin molecule or the fragment thereof is selected from any one of
- Laminin-111 or a fragment thereof that adheres to the substrate at an amount of 0.15 μg/cm2 or more,
- Laminin-211 or a fragment thereof that adheres to the substrate at an amount of 0.52 μg/cm2 or more,
- Laminin-221 or a fragment thereof that adheres to the substrate at an amount of 0.34 μg/cm2,
- Laminin-311 or a fragment thereof that adheres to the substrate at a maximum amount after air drying of 0.5 μg/cm2 or more,
- Laminin-332 or a fragment thereof that adheres to the substrate at a maximum amount after air drying of 1.2 μg/cm2 or more,
- Laminin-421 or a fragment thereof that adheres to the substrate at an amount of 0.50 μg/cm2 or more,
- Laminin-511 or a fragment thereof that adheres to the substrate at an amount of 0.32 μg/cm2 or more,
- a variant of E8 region of Laminin-511 that adheres to the substrate at an amount of 0.15 μg/cm2 or more, and
- Laminin-521 or a fragment thereof that adheres to the substrate at an amount of 0.44 μg/cm2 or more.
11 more elements
Dependencies are inferred from the claim text.